Identification of bacterial guanylate cyclases
نویسندگان
چکیده
منابع مشابه
Characterization of particulate and soluble guanylate cyclases from rat lung.
Rat lung homogenates contained significant amounts of guanylate cyclase activity in both 100,000 times g (60 min) particulate and supernatant fractions. In the presence of detergent, the particulate fraction contained 40% as much activity as did the supernatant fraction. Detergent-dispersed particulate and partially purified soluble guanylate cyclase preparations were characterized with respect...
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This monograph presents a historical perspective of cornerstone developments on the biochemistry and physiology of mammalian membrane guanylate cyclases (MGCs), highlighting contributions made by the authors and their collaborators. Upon resolution of early contentious studies, cyclic GMP emerged alongside cyclic AMP, as an important intracellular second messenger for hormonal signaling. Howeve...
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Adenylate and guanylate cyclases were assayed in silkmoth fat body homogenates by measuring the conversion of [cc-3tP]nucleoside triphosphates to cyclic [32P]nucleotides. Adenylate cyclase was dependent on dithiothreitol, required either Mg2’ or MI?+ for activity, was activated by NaF, and inhibited by Triton X-100. Guanylate cyclase was not dependent on dithiothreitol, was strictly dependent u...
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Over 30 receptor-like kinases contain a guanylate cyclase (GC) catalytic centre embedded within the C-terminal region of their kinase domain in the model plant Arabidopsis. A number of the kinase GCs contain both functional kinase and GC activity in vitro and the natural ligands of these receptors stimulate increases in cGMP within isolated protoplasts. The GC activity could be described as a m...
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The products obtained by incubation of farnesyl diphosphate (FPP) with six purified bacterial terpene cyclases were characterised by one- and two-dimensional NMR spectroscopic methods, allowing for a full structure elucidation. The absolute configurations of four terpenes were determined based on their optical rotary powers. Incubation experiments with 13C-labelled isotopomers of FPP in buffers...
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ژورنال
عنوان ژورنال: Proteins: Structure, Function, and Bioinformatics
سال: 2017
ISSN: 0887-3585
DOI: 10.1002/prot.25308